DNA

Part:BBa_K4812004:Design

Designed by: Rashik Chand   Group: iGEM23_NYU-Abu-Dhabi   (2023-10-08)


Scl2.28-1_Fragment 3


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 18
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence has been codon optimized for E.Coli K12.


Source

Scl2.28-1 allele comes from the cell surface of group A Streptococcus(GAS), a Gram-positive bacterium, specifically serotype M28 strain MGAS6274.

References